Experimental study on reduction of U (Ⅵ) by an anaerobic bacterium, Shewanella putrefaciens: Application to sandstone-hosted interlayer oxidation-zone type uranium deposits, China

An experimental study on reduction of U (Ⅵ) by anaerobic bacteria, Shewane//a putrefaciens, is first reported here in China. The experimental conditions were: 35℃ and pH =7.0-7.4, corresponding to a physicochemical environments in which the sandstone-hosted interlayer oxidation-zone type uranium deposit formed in Northwest China's Xinjiang. Bacteria adopted in the present experiment, Shewanella putrefaciens, occur extensively in natural environment. Our study shows that nano-crystal precipitates of uraninite quickly occurred on the surface of the cells within one week. It was found that the pitchblende was characterized by a random arrangement of uraninite nanocrystals (2-4 nm) in it, significantly different from natural pitchblende in which uraninite nanocrystals are arranged in order. Finally, a possible mechanism of uranium biomineralization by microorganisms in the deposits is discussed. Our investigation may supply a technical train of thoughts for bioremediation of nuclear-contaminated water environments and for underground dissolving extraction of the sandstone-hosted uranium ores.     

Biomineralization of Uranium: A Simulated Experiment and Its Significance

A simulated experimental reduction of U^v1 and the synthesis of uraninite by a sulfate-reducing bacteria,Desulfovibrio desulfuricans DSM 642, are first reported. The simulated physicochemical experimental conditions were:35℃, pH=7.0-7.4, corresponding to the environments of formation of the sandstone-hosted interlayer oxidation-zone type uranium deposits in Xinjiang, NW China. Uraninite was formed on the surface of the host bacteria after a one-week‘s incubation. Therefore, sulfate-reducing bacteria, which existed extensively in Jurassic sandstone-producing environments,might have participated in the biomineralization of this uranium deposit. There is an important difference in the orderdisorder of the crystalline structure between the uraninite produced by Desulfovibrio desulfuricans and naturally occurring uraninite. Long time and slow precipitation and growth of uraninite in the geological environment might have resulted in larger uraninite crystals, with uraninite nanocrystals arranged in order, whereas the experimentally produced uraninite is composed of unordered uraninite nanocrystals which, in contrast, result from the short time span of formation and rapid precipitation and growth of uraninite. The discovery has important implications for understanding genetic significance in mineralogy, and also indicates that in-situ bioremediation of U-contaminated environments and use of biotechnology in the treatment of radioactive liquid waste is being contemplated.     

甘肃北山野马泉岩体同位素地球化学特征

Nd、Sr、Pb同位素研究结果揭示：甘肃北山野马泉岩体第Ⅰ、Ⅱ侵入阶段花岗岩类具有Ⅰ-型花岗岩的特征，(87Sr/86Sr)i=0.708~0.710，εNd=-2.229~-5.866，在207 Pb/204Pb-206Pb/204Pb构造模式图上其投影点落在造山带演化线附近，在εNd-εSr图解中，其投影点落人Ⅰ-型花岗岩类范围内，其成岩物质为壳幔混合来源。第Ⅲ侵人阶段的岩石成因类型为S-型，其(87Sr/86Sr)i=0.7149~0.7358, εNd＝-7.3750~-8.9556。该岩体形成的地球动力学环境是北山陆内碰撞造山带。     

用随机有限断层方法模拟2016-11-13新西兰Mw7.8地震

在未来大地震发震构造已知的前提下，提出用经验公式确定大地震断层长度、宽度、地震矩和滑动分布等参数，并用随机有限断层方法预测未来大地震。用随机有限断层方法模拟2016-11-13新西兰M_W7.8地震的12个基岩台站地震动时程和反应谱，并用模拟的频谱幅值与记录频谱幅值平均比值确定模拟误差。结果表明，在周期为0~10 s的范围内，模拟误差在0.92~1.08之间，不同频率模拟误差的标准差变化范围不超过1。95%的置信区间宽度随频率无明显变化，模拟结果反映了地震动记录的平均效果。用准随机方法重新获取新西兰大地震断层初始破裂点和位错滑动分布，模拟地震动并计算模拟误差随频率的变化，得到与最初模型相似的结论，从而进一步证实了本文提出的获取震源参数并用随机有限断层方法预测未来大地震的可靠性，且其特别适用于大地震远场模拟。     

流域尺度土地利用调蓄视角的雨洪管理探析

发达国家雨洪管理焦点历经管网设计、不透水面阈值控制、低影响开发及土地利用管理等过程,呈现“多尺度多视角衔接”趋势。本文聚焦太湖流域土地利用调蓄功能变化机制及管理应用,揭示了1985-2015年来建设占用耕地是调蓄功能降低的主要原因;发现基于小流域的调蓄功能变化与建设用地强度整体低关联,但局部高相关,高相关的地区位于大城市或某些乡镇周边的建设增长热区,而这些热区的建设用地与调蓄空间规模较大且接触机会更多,是开发之前调蓄空间保护、开发控制以及开发之后综合管理等3个土地利用调蓄目标因子协调不当的结果;最后提出流域尺度土地利用调蓄视角的雨洪管理“345”模式,即以3个目标因子和5类控制要素为基础,实施土地利用调蓄创建、防御、拓展与保护等4类差别化战略模式,从更大尺度认识老城区和新区的雨洪关联,拓展海绵城市建设的本土认知和视野。     

黄连素对Aβ25-35诱导的星形胶质细胞的细胞因子及氧化应激的影响

目的：探讨黄连素对β-淀粉样蛋白25-35（Aβ25-35）诱导的大鼠星形胶质细胞的细胞因子及氧化应激的影响。方法：培养第3代的大鼠星形胶质细胞，将其分为空白对照组、模型组、多奈哌齐组，以及黄连素低、高剂量组，分别加入相应药物培养24 h后，模型组和各药物组再加入Aβ₂₅-35。检测各组IL-1β、IL-6、TNF-α mRNA的表达及SOD、MDA水平。结果：Aβ₂₅-35浓度为20 μmol/L时星形胶质细胞增殖率最高；与空白组比较，模型组的IL-1β、IL-6、TNF-α蛋白分泌及其mRNA表达增多，伴随着MDA水平上升，SOD水平下降；与模型组比较，各药物组的IL-1β、IL-6、TNF-α蛋白分泌及其mRNA表达减少，伴随着MDA水平下降，SOD水平上升，尤以黄连素高剂量组疗效最好。结论：黄连素可降低Aβ₂₅-35诱导的大鼠星形胶质细胞的炎性细胞因子，并减少氧化应激。     

Anti-inflammatory and Immunomodulatory Effects of Marine n-3 Polyunsaturated Fatty Acids on Human Health and Diseases

The pharmaceutical effects of n-3 polyunsaturated fatty acids(n-3 PUFAs) as dietary nutrients on human health and diseases have gained much attention and are investigated for decades. Docosahexaenoic acid(DHA), eicosapentaenoic acid(EPA) and docosapentaenoic acid(DPA) are the three major n-3 PUFAs enriched in marine organisms, such as fish, shrimp, algae, and so on. It has been well known that n-3 PUFAs, especially DHA and EPA, are beneficial in reducing the risk of cardiovascular and cerebrovascular diseases. Accumulating evidence suggests that n-3 PUFAs might cure inflammatory diseases through several mechanisms, such as plasma membrane remodeling of lymphocytes, down-regulating pro-inflammatory cytokines, and alternating adhesion molecule expressions. Several molecular targets of n-3 PUFAs on immune-regulation have also been identified, such as GPR120(FFA4), protein kinase C(PKC), and PPAR-γ. However, it remains inconclusive if dietary n-3 PUFAs function the same both in vitro and in vivo based on cohort studies. This review will focus on the molecular targets and mechanisms of anti-inflammatory and immunomodulatory effects of n-3 PUFAs on human health and diseases, such as obesity, tumor, diabetes, and autoimmune diseases.     

Characterization of Turbot(Scophthalmus maximus) Skin and the Extracted Acid-Soluble Collagen

The biochemical composition of the turbot skin was investigated. The moisture level of the skin was found to be 51.4%. Based on dry matter content, there were relatively high protein(82.1%) and lipid(13.1%) concentrations in the turbot skin. Mineral element analysis revealed that the turbot skin had high Ca content(2069.0 mg kg-1), and the concentrations of toxic heavy metals Hg and Pb were less than 0.005 mg kg-1, which indicates that the turbot skin is a safe resource for collagen production. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis showed that acid-soluble collagen(ASC) extracted from the turbot skin was type-I collagen. The imino acid content of the ASC was 241.6 per 1000 amino acids, which suggests a relatively high denaturation temperature. The Fourier transform infrared(FTIR) spectra of ASC reflected a highly stable structure, and the measured denaturation temperature of ASC was 29.5℃, which is higher than those from many temperate fishes. ASC was the most soluble at pH 4.0, and when the pH values were below or above 4.0, the solubility decreased rapidly. The ASC exhibited a relatively high solubility when NaCl concentration was lower than 2%. These results show that turbo skin can be employed as a source for producing collagen with high quality.     

城市居民压力源对幸福感的影响研究——基于乡村旅游休闲参与的角度

居民幸福感研究日益受到重视,以往研究多是从自然环境、社会人文环境和城市化进程等方面探讨大尺度空间环境因素对城市居民幸福感的影响。结合乡村旅游的角度探讨城市居民幸福感的影响研究,目前还较少。运用SEM分析方法,探究处于多维压力之下的城市居民旅游者如何通过前往乡村进行旅游休闲活动提升幸福感,进行模型构建与分析,结果显示：① 压力源对调适策略有显著正向影响,对休闲参与不显著,但不同群体影响差异显著,压力调适对休闲参与有显著正向影响;② 休闲参与对心流体验和幸福感具有显著正向关系,且心流体验对幸福感具有显著正向影响;③ 心流体验对休闲效益具有正向显著影响,且休闲效益对幸福感具有正向显著影响;④ 心流体验在休闲参与对幸福感、休闲效益在心流体验对幸福感均具有显著中介效应;⑤ 旅游者不同背景变项在压力调适、休闲参与及幸福感等分别呈现不同程度的显著差异。     

城市点云的区域生长三角网构建方法

成熟的倾斜摄影测量及密集匹配技术可获取密集的城市点云,对点云进行三角网构建是真三维建模的关键技术之一。提出了基于区域生长的三角网构建方法,使用双约束条件提高点云的搜索效率,基于点边表数据结构进行网格生长,利用夹角约束和三角形孔洞修补优化网格。实验证明,该方法获取的三角网能够准确地表达楼体、桥状建筑等在内的城市实体,网格优化后的孔洞比例约为1‰并且该方法具有一定的抗噪能力。